Clinical and Experimental Pharmacology Group - Fully Validated Assays

 

Laboratory Method SOPs 
(Full Descriptions of CEP Validated Methods)

Status

The M30 Elisa assay for caspase CK18

Issued

 

The M65 Elisa Assay for cytokeratin 18

Issued

 

 RH1 in Plasma by HPLC-MS-MS

Issued

 

Extraction of protein from patient samples

Issued

 

SDS PAGE for XIAP in patient samples

Issued

 

Western blot analysis of XIAP Protein

Issued

 

RNA extraction from patient samples

Issued

 

q RT-PCR for XIAP mRNA 

Issued

 

Comet-X assay for DNA crosslinks

Issued

 

RFLP for DT-Diaphorase SNPs

Issued

 

DCPIP for  DT-Diaphorase  activity

Issued

 

IHC for DT-Diaphorase

Issued

 

Western blot for DT-Diaphorase

Issued

 

Cell death detection ELISA for nDNA

Issued

 

Generic Fast track ELISA validation

Issued

 

IHC for Bcl-2 in Human tumour/ tissue

Issued

 

IHC for Bcl/xL

Issued

 

IHC for Mcl-1

Issued

 

IHC for Cleaved Caspase 3

Issued

 

Rituximab ELISA

Issued

 

ELISA for HAMA

Issued

 

IHC for CD31 and MVD counts

Issued

 

IHC for CD 34 and MVD counts

Issued

 

IHC for CD105 and MVD counts

Issued

 

LC/MS for GSAO in plasma

Issued

 

CD105 ELISA

Issued

 

VEGF-A ELISA

Issued

 

PIGF ELISA

Issued

 

VEGF-R1 ELISA

Issued

 

VEGF-R2 ELISA

Issued

 

Two colour analysis of apoptotic cells by FACSArray

Issued

 

Three colour analysis of apoptotic cells by FACSArray

Issued

 

Detection of Circulating Tumour Cells using CellSearch

Issued

 

Ang 1 ELISA

 

Draft

Ang 2 ELISA

 

Draft

Tie 2 ELISA

 

Draft

VEGF-D ELISA

 

Draft

SearchLight Plus multiplex ELISA method

Issued

 

Four colour analysis of apoptotic cells by FACSArray

Issued

 

Coating efficiency for Rituximab ELISA

Issued

 

PDGF-BB ELISA

 

Draft

bFGF ELISA

 

Draft

IL-8 ELISA

 

Draft

SDF-1 ELISA

 

Draft

HGF ELISA

 

Draft

Osteopontin ELISA

 

Draft

Laboratory Method SOPs 
(Full Descriptions of CEP Validated Methods)

Status

 

TIE-2 ELISA

 

Draft

DNA extraction from plasma/serum using a QIAamp spin kit

 

Draft

DxS PI3kinase mutation  test kit

 

Draft

CYFRA 21-1 ELISA

 

Draft

Flt-3 ELISA

 

Draft

 

Group Leader:
Caroline Dive

After completing my PhD studies in Cambridge, I moved to Aston University's School of Pharmaceutical Sciences in Birmingham where I started my own group studying mechanisms of drug induced tumour cell death. I then moved to what became the Faculty of Life Sciences at The University of Manchester to continue this research. I was awarded a Lister Institute of Preventative Medicine Research Fellowship before moving to the Paterson Institute for Cancer Research in 2003.  Here I set up the Clinical and Experimental Pharmacology Group interfacing with the Derek Crowther Unit for early clinical trials at The Christie. I am currently a Senior Group Leader at the Paterson Institute for Cancer Research and Professor of Pharmacology at The University of Manchester.

Deputy Group Leader 
Gerard Brady

Disease Focus Team Leaders 
Fiona Blackhall
Guy Makin
Andrew Renehan

Staff Scientists
Jeff Cummings
Dominic Rothwell
Jonathan Tugwood

Clinical Lecturers
Emma Dean
Alastair Greystoke
Matthew Krebs
Cliona Kirwan

Associate Scientists
Kathryn Simpson
Chris Morrow

Service Manager
David Moore

Laboratory Manager
Martin Greaves

Postdoctoral Fellows
Alison Backen
Becky Bola
Radoslaw Polanski
Francesca Trapani
Cong Zhou

Clinical Fellows
Kyaw Aung
Louise Carter
Leila Khoja
Kalena Marti Marti
Robert Metcalf
Danielle Shaw
Laura Cove Smith

Graduate Student
Danielle Potter

Scientific Officers
Mahmood Ayub
Jenny Antonello
Stephen Bramley
Debbie Burt
Fouziah Butt
John Castle
Samson Chinien
Jakub Chudziak
Martin Dawson
Olive Denneny
Suzanne Faulkner
Joseph Halstead
Grace Hampson
Cassandra Hodgkinson
Paul Kelly
Matthew Lancashire
Daniel Morris
Karen Morris
Jackie Pierce
Lynsey Priest
Tony Price
Robert Sloane
Nigel Smith

Scientific Assistant
Aileen Jardine

Admin Assistant
Lisa Waters