Biological Mass Spectrometry Facility

Enhanced detection of ubiquitin isopeptides using reductive methylation. 
Chicooree N, Connolly Y, Tan CT, Malliri A, Li Y, Smith DL, Griffiths JR.
J Am Soc Mass Spectrom. 2013 Mar;24(3):421-30. doi: 10.1007/s13361-012-0538-0. Epub 2013 Jan 30. PMID: 23361369 [PubMed - in process]

A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine. 
Chicooree N, Griffiths JR, Connolly Y, Tan CT, Malliri A, Eyers CE, Smith DL.
Rapid Commun Mass Spectrom. 2013 Jan 15;27(1):127-34. doi: 10.1002/rcm.6425. PMID: 23239325 [PubMed - in process]

The utility of porous graphitic carbon as a stationary phase in proteomics workflows: Two-dimensional chromatography of complex peptide samples.
Griffiths JR, Perkins S, Connolly Y, Zhang L, Holland M, Barattini V, Pereira L, Edge A, Ritchie H, Smith DL.
J Chromatogr A. 2012 Jan 13. [Epub ahead of print] PMID: 22326183 [PubMed - as supplied by publisher]

Proteomic Plasma Membrane Profiling Reveals an Essential Role for gp96 in the Cell Surface Expression of LDLR Family Members, Including the LDL Receptor and LRP6.
Weekes MP, Antrobus R, Talbot S, Hör S, Simecek N, Smith DL, Bloor S, Randow F, Lehner PJ.
J Proteome Res. 2012 Feb 7. [Epub ahead of print] PMID: 22292497 [PubMed - as supplied by publisher]

Cell Cycle Group

Eukaryotic replisome components cooperate to process histones during chromosome replication 
Foltman, M., Evrin, C., De Piccoli, G., Jones, R.C., Edmondson, R.D., Katou, Y., Nakato, R., Shirahige, K., and Labib, K. (2013). Cell Rep., 3, 892-904.

Dpb2 integrates the leading strand DNA polymerase into the eukaryotic replisome.
Sengupta, S., van Deursen, F., De Piccoli, and Labib, K. (2013). Curr. Biol., 23, 543-552.

Hof1 and Rvs167 Have Redundant Roles in Actomyosin Ring Function during Cytokinesis in Budding Yeast. 
Nkosi PJ, Targosz BS, Labib K, Sanchez-Diaz A.
PLoS One. 2013;8(2):e57846. doi: 10.1371/journal.pone.0057846. Epub 2013 Feb 28. PMID: 23469085 [PubMed - in process]

Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts.
Devrekanli A, Foltman M, Roncero C, Sanchez-Diaz A, Labib K.
J Cell Sci. 2012 Sep 6. [Epub ahead of print]

The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth.
Sanchez-Diaz A, Nkosi PJ, Murray S, Labib K. (2012) EMBO J.,
doi: 10.1038/emboj.2012.224. [Epub ahead of print].

A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome. J. Biol. Chem., 2012 May 16.
Kilkenny, M.L., De Piccoli, G., Perera, R.L., Labib, K., Pellegrini, L. (2012). [Epub ahead of print].

Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation.
van Deursen F, Sengupta S, De Piccoli G, Sanchez-Diaz A, Labib K.
EMBO J. 2012 Mar 20. doi: 10.1038/emboj.2012.69. [Epub ahead of print]

Replisome Stability at Defective DNA Replication Forks Is Independent of S Phase Checkpoint Kinases
De Piccoli, G., Katou, Y., Itoh, T., Nakato, R., Shirahige, K., and Labib, K.
Mol. Cell, 2012,10.1016/j.molcel.2012.01.007 [Epub ahead of print].

Cell Division Group

Removal of Centrosomal PP1 by NIMA Kinase Unlocks the MPF Feedback Loop to Promote Mitotic Commitment in S. pombe.
Grallert A, Chan KY, Alonso-Nuñez ML, Madrid M, Biswas A, Alvarez-Tabarés I, Connolly Y, Tanaka K, Robertson A, Ortiz JM, Smith DL, Hagan IM.
Curr Biol. 2013 Feb 4;23(3):213-22. doi: 10.1016/j.cub.2012.12.039. Epub 2013 Jan 17. PMID: 23333317 [PubMed - in process]

The S. pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1.
Grallert A, Connolly Y, Smith DL, Simanis V, Hagan IM.
Nat Cell Biol. 2012 Jun 10;14(7):738-45.

Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast.
Grallert A, Patel A, Tallada VA, Chan KY, Bagley S, Krapp A, Simanis V, Hagan IM.
Nat Cell Biol. 2012 Dec 9. doi: 10.1038/ncb2633. [Epub ahead of print] PMID:23222840[PubMed - as supplied by publisher]

Cell Regulation Group

Sensitisation of c-MYC-induced B-lymphoma cells to apoptosis by ATF2.
Walczynski J, Lyons S, Jones N, Breitwieser W.
Oncogene. 2013 Feb 18. doi: 10.1038/onc.2013.28. [Epub ahead of print] PMID: 23416976 [PubMed - as supplied by publisher]

The roles of ATF2 (activating transcription factor 2) in tumorigenesis.
Gozdecka M, Breitwieser W.
Biochem Soc Trans. 2012 Feb 1;40(1):230-4.

Cell Signalling Group

Constitutive Rac Activation Is Not Sufficient to Initiate Melanocyte Neoplasia but Accelerates Malignant Progression.
Dalton LE, Kamarashev J, Barinaga-Rementeria Ramirez I, White G, Malliri A, Hurlstone A.
J Invest Dermatol. 2013 Jan 21. doi: 10.1038/jid.2013.23. [Epub ahead of print] PMID: 23337888 [PubMed - as supplied by publisher]

he tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation.
Castillo-Lluva S, Tan CT, Daugaard M, Sorensen PH, Malliri A.
Oncogene. 2012 May 21. doi: 10.1038/onc.2012.189. [Epub ahead of print]

β2-syntrophin and Par-3 promote an apicobasal Rac activity gradient at cell-cell junctions by differentially regulating Tiam1 activity.
Mack NA, Porter AP, Whalley HJ, Schwarz JP, Jones RC, Khaja AS, Bjartell A, Anderson KI, Malliri A.
Nat Cell Biol. 2012 Nov;14(11):1169-80. doi: 10.1038/ncb2608. Epub 2012 Oct 28. PMID:23103911[PubMed - in process]

Clinical and Experimental Pharmacology Group

Biomarker Utility of Circulating Tumor Cells in Metastatic Cutaneous Melanoma.
Khoja L, Lorigan P, Zhou C, Lancashire M, Booth J, Cummings J, Califano R, Clack G, Hughes A, Dive C.
J Invest Dermatol. 2012 Dec 6. doi: 10.1038/jid.2012.468. [Epub ahead of print] PMID: 23223143 [PubMed - as supplied by publisher]

Biology and clinical relevance of circulating tumour cells.
Carter L, Metcalf R, Blackhall FH, Dive C, Krebs MG.
J Thorac Dis. 2012 Oct;4(5):453-5. doi: 10.3978/j.issn.2072-1439.2012.09.02. No abstract available. PMID: 23050105 [PubMed - in process]

Multi-level evidence that circulating CK18 is a biomarker of tumour burden in colorectal cancer.
Greystoke A, Dean E, Saunders MP, Cummings J, Hughes A, Ranson M, Dive C, Renehan AG.
Br J Cancer. 2012 Sep 20. doi: 10.1038/bjc.2012.416. [Epub ahead of print]

Evaluation of cell death mechanisms induced by the vascular disrupting agent OXi4503 during a phase I clinical trial.
Cummings J, Zweifel M, Smith N, Ross P, Peters J, Rustin G, Price P, Middleton MR, Ward T, Dive C. 
Br J Cancer. 2012 Apr 26. [Epub ahead of print].

Clinical Significance and Molecular Characteristics of Circulating Tumor Cells and Circulating Tumor Microemboli in Patients With Small-Cell Lung Cancer.
Hou JM, Krebs MG, Lancashire L, Sloane R, Backen A, Swain RK, Priest LJ, Greystoke A, Zhou C, Morris K, Ward T, Blackhall FH, Dive C.
J Clin Oncol. 2012 Jan 17. [Epub ahead of print]

A Pilot Study Assessing the Prognostic Value of CK18 and nDNA Biomarkers in Severe Sepsis Patients.
Moore DJ, Greystoke A, Butt F, Wurthner J, Growcott J, Hughes A, Dive C.
Clin Drug Investig. 2012 Jan 4. [Epub ahead of print]

DNA Damage Response Group

Deficiency of terminal ADP-ribose protein glycohydrolase TARG1/C6orf130 in neurodegenerative disease.
Sharifi, R., Morra, R., Appel, C., Tallis, M., Chioza, B., Jankevicius, G., Simpson, M.A., Matic, I., Ozkan, E., Golia, B., Schellenberg, M.J., Weston, R., Williams, J.G., Rossi, M.N., Galehdari, H., Krahn, J., Wan, A., Trembath, R.C., Crosby, A.H., Ahel, D., Hay, R., Ladurner, A.G., Timinszky, G., Williams, R.S. & Ahel, I. (2013).  EMBO J. (Epub ahead of print).

Molecular Insights into Poly(ADP-ribose) Recognition and Processing
Žaja R, Mikoc A, Barkauskaite E, Ahel I.
Biomolecules 2013, 3(1), 1-17; doi:10.3390/biom3010001

Structure and mechanism of a canonical poly(ADP-ribose) glycohydrolase.
Dunstan MS, Barkauskaite E, Lafite P, Knezevic CE, Brassington A, Ahel M, Hergenrother PJ, Leys D, Ahel I.
Nat Commun. 2012 Jun 6;3:878. doi: 10.1038/ncomms1889.

ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response.
Weston R, Peeters H, Ahel D.
Genes Dev. 2012 Jul 15;26(14):1558-72.


Drug Discovery Group

Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.
Thomson G, Watson A, Caldecott K, Denneny O, Depledge P, Hamilton N, Hopkins G, Jordan A, Morrow C, Raoof A, Waddell I, Ogilvie D.
Anal Biochem. 2013 Feb 12. doi:pii: S0003-2697(13)00073-0. 10.1016/j.ab.2013.02.001. [Epub ahead of print] PMID: 23416181 [PubMed - as supplied by publisher]

Novel Steroid Inhibitors of Glucose 6-Phosphate Dehydrogenase.
Hamilton NM, Dawson M, Fairweather EE, Hamilton NS, Hitchin JR, James DI, Jones SD, Jordan AM, Lyons AJ, Small HF, Thomson GJ, Waddell ID, Ogilvie DJ.
J Med Chem. 2012 Apr 27. [Epub ahead of print].

Immunology Group

Therapy of human papillomavirus-related disease.
Stern PL, van der Burg SH, Hampson IN, Broker TR, Fiander A, Lacey CJ, Kitchener HC, Einstein MH.
Vaccine. 2012 Nov 20;30 Suppl 5:F71-82. doi: 10.1016/j.vaccine.2012.05.091. PMID: 23199967 [PubMed - in process]

CXCL12 receptor preference, signal transduction, biological response and the expression of 5T4 oncofoetal glycoprotein.
McGinn OJ, Marinov G, Sawan S, Stern PL.
J Cell Sci. 2012 Sep 6. [Epub ahead of print]

5T4 oncofoetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype.
Castro FV, McGinn OJ, Krishnan S, Marinov G, Li J, Rutkowski AJ, Elkord E, Burt D, Holland M, Vaghjiani R, Gallego A, Saha V, Stern PL.
Leukemia. 2012 Jan 23. doi: 10.1038/leu.2012.18. [Epub ahead of print]


Inositide Group

Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis.
Lekomtsev S, Su KC, Pye VE, Blight K, Sundaramoorthy S, Takaki T, Collinson LM, Cherepanov P, Divecha N, Petronczki M.
Nature. 2012 Dec 13;492(7428):276-9. doi: 10.1038/nature11773. PMID:23235882[PubMed - in process]

Regulation of phosphatidylinositol-5-phosphate signaling by pin1 determines sensitivity to oxidative stress.
Keune WJ, Jones DR, Bultsma Y, Sommer L, Zhou XZ, Lu KP, Divecha N.
Sci Signal. 2012 Nov 27;5(252):ra86. doi: 10.1126/scisignal.2003223. PMID:23193159[PubMed - in process]

Diacylglycerol kinase θ counteracts protein kinase C-mediated inactivation of the EGF receptor.
van Baal J, de Widt J, Divecha N, van Blitterswijk WJ.
Int J Biochem Cell Biol. 2012 Nov;44(11):1791-9. doi: 10.1016/j.biocel.2012.06.021. Epub 2012 Jun 23. PMID:22732145[PubMed - in process]

Nuclear phosphoinositides: location, regulation and function.
Fiume R, Keune WJ, Faenza I, Bultsma Y, Ramazzotti G, Jones DR, Martelli AM, Somner L, Follo MY, Divecha N, Cocco L.
Subcell Biochem. 2012;59:335-61. doi: 10.1007/978-94-007-3015-1_11. PMID:22374096[PubMed - in process]

Divergent functions of the myotubularin (MTM) homologs AtMTM1 and AtMTM2 in Arabidopsis thaliana: evolution of the plant MTM family.   
Ding Y, Ndamukong I, Zhao Y, Xia Y, Riethoven JJ, Jones DR, Divecha N, Avramova Z.
Plant J. 2012 Jun;70(5):866-78. doi: 10.1111/j.1365-313X.2012.04936.x. Epub 2012 Mar 30. PMID:22324391[PubMed - indexed for MEDLINE]

Impaired neural development in a zebrafish model for Lowe syndrome.
Ramirez IB, Pietka G, Jones DR, Divecha N, Alia A, Baraban SC, Hurlstone AF, Lowe M.
Hum Mol Genet. 2012 Apr 15;21(8):1744-59. doi: 10.1093/hmg/ddr608. Epub 2011 Dec 30.
PMID:22210625[PubMed - indexed for MEDLINE]


Leukaemia Biology Group

LSD1 inhibition: a therapeutic strategy in cancer?
Lynch JT, Harris WJ, Somervaille TC.
Expert Opin Ther Targets. 2012 Sep 8. [Epub ahead of print]

The Histone Demethylase KDM1A Sustains the Oncogenic Potential of MLL-AF9 Leukemia Stem Cells.
Harris WJ, Huang X, Lynch JT, Spencer GJ, Hitchin JR, Li Y, Ciceri F, Blaser JG, Greystoke BF, Jordan AM, Miller CJ, Ogilvie DJ, Somervaille TC. 
Cancer Cell. 2012 Mar 28. [Epub ahead of print]

Molecular Oncology

Inhibiting EGF Receptor or SRC Family Kinase Signaling Overcomes BRAF Inhibitor Resistance in Melanoma.
Girotti MR, Pedersen M, Sanchez-Laorden B, Viros A, Turajlic S, Niculescu-Duvaz D, Zambon A, Sinclair J, Hayes A, Gore M, Lorigan P, Springer C, Larkin J, Jorgensen C, Marais R.
Cancer Discov. 2013 Feb;3(2):158-67. doi: 10.1158/2159-8290.CD-12-0386. Epub 2012 Dec 14. PMID: 23242808 [PubMed - in process]

Stem Cell Biology Group

The MYSTerious MOZ, a Histone Acetyltransferase with a Key Role in Haematopoiesis.
Perez-Campo FM, Costa G, Lie-A-Ling M, Kouskoff V, Lacaud G.
Immunology. 2013 Jan 24. doi: 10.1111/imm.12072. [Epub ahead of print] PMID: 23347099 [PubMed - as supplied by publisher]

GFI1 and GFI1B control the loss of endothelial identity of hemogenic endothelium during hematopoietic commitment.
Lancrin C, Mazan M, Stefanska M, Patel R, Lichtinger M, Costa G, Vargel O, Wilson NK, Möröy T, Bonifer C, Göttgens B, Kouskoff V, Lacaud G.
Blood. 2012 Jul 12;120(2):314-22. 


Stem Cell Haematopoiesis Group

The Flk1-cre Mediated Deletion of ETV2 Defines Its Narrow Temporal Requirement During Embryonic Hematopoietic Development.
Wareing S, Mazan A, Pearson S, Göttgens B, Lacaud G, Kouskoff V.
Stem Cells. 2012 May 8. [Epub ahead of print].

ETV2 expression marks blood and endothelium precursors, including hemogenic endothelium, at the onset of blood development.
Wareing S, Eliades A, Lacaud G, Kouskoff V.
Dev Dyn. 2012 Jun 25. doi: 10.1002/dvdy.23825. [Epub ahead of print]